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Become A Master in HPLC Technique - (Liquid Chromatography)


High Performance Liquid Chromatography (HPLC) is the most commonly used analytical tool in testing laboratories related to Analytical Chemistry field, allowing for the separation, identification, purification and quantification of drug substances or any organic compound.

In fact, High performance liquid chromatography is an incredible technique with wide applications in Analytical Chemistry, the food industry, pharmaceutical field, research of new compounds in universities and colleges and many other areas.

For students who complete training in HPLC, it’s possible to secure a great career in a number of exciting fields:

  • R&D Scientists
  • QA Managers & Practicing Chemists
  • Students in disciplines of Chemistry,  Pharmacy, Food &  Nutrition, Polymer Science, and Agricultural Sciences.
  • Life Science researchers
  • Governmental Regulatory Bodies
  • Environmental Science

The operation of HPLC is the required fundamental skill for all chemists and technicians using HPLC.

Recent industry and student surveys found that there is a high training demand for basic HPLC operation skills for diploma graduates and working adults intending to work in the testing laboratories.  However, many of these laboratories do not have enough resources for staff training.  This course aims to assist the industry in training its prospective and existing employees the basic skills for the operation of HPLC. It provides a comprehensive review of basic concepts of High performance Liquid Chromatography.

This course will help you in covering everything you will need to know about HPLC and How it works. The components of this fantastic instrument and the Chemistry of the heart of HPLC which is the stationary phase(The Column).  It doesn’t matter how much, or how little, prior knowledge of Liquid Chromatography you’ve got as this course will take you through all the necessary stages.

Am  Mohammad Abualrub, I have PhD in Chemistry and am Expert in pharmaceutical Industry – +8Years & Expert in teaching Chemistry +4Years. I have been using HPLC for more than 8 Years!!!

This course covers the following topics:

  1. Introduction to Liquid Chromatography
  2. How HPLC Works
  3. HPLC Analysis
  4. Components of HPLC
  5. Column Chemistry – Stationary Phase
  6. System Suitability
  7. Starting HPLC System
  8. Determination The Assay Using HPLC
  9. Method Development

You will get the opportunity to see a real HPLC instrument inside the  analytical chemistry lab in my College!!!

See how I run HPLC system, installing the Column, and preparing the Mobile Phase, all this will be done in the lab !!! So what you are waiting ???

Test Your Basic General learning about HPLC System in This Quiz:

  1. HPLC stands for
  2. Typical HPLC column dimensions are
  3. Pressure range in normal analytical separations is
  4. The mobile phase used in HPLC analysis is
  5. Increase in flow rate of mobile phase leads to
  6. HPLC columns are generally made of
  7. Most commonly used HPLC detector is
  8. Most commonly used mode of HPLC analysis is
  9. Isocratic mode of operation implies
  10. Retention time drifts of eluting compounds are controlled by

Who this course is for:

  • Students in disciplines of Chemistry, Pharmacy, Food & Nutrition, Polymer Science, and Agricultural Sciences.
  • R&D Scientists and employees.
  • QA Managers & Practicing Chemists, QC employees.
  • Life Science researchers and students.
  • Governmental Regulatory Bodies.
  • Environmental Science Students.
  • Interested Students To Join the Pharmaceutical Field.


  • Understanding The Basics of Analytical Chemistry.
  • Desire to learn more about HPLC and a willingness to make the necessary effort.

Last updated 10/2018


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